Fig. 1

Elevation of histone crotonylation level in the peripheral blood mononuclear cells (PBMCs) of colorectal cancer (CRC) patients. A Identification of lysine crotonylation (Kcr) presented in the PBMCs of healthy controls and CRC patients using anti-crotonylation antibody. The protein loading amount in each lane was 20 µg extracted from the lysates of PBMCs. The SDS–PAGE gel stained with Coomassie brilliant blue showed the presence of proteins. B Western blots of histone crotonylation of the protein lysates of PBMCs from healthy controls and CRC patients (n = 40). Anti-histone H3 antibody was used as the loading control. Results were expressed as percentage change of crotonylated histones over control. The intensity of Kcr was quantified using ImageJ software, followed by statistical analysis. Each scale bar represents the mean ± SD of the 40 samples. The means were normalized to H3 (*P < 0.05 vs. healthy controls)