Fig. 3
From: Altered cfDNA fragmentation profile in hypomethylated regions as diagnostic markers in breast cancer
Short cfDNA fragments ratio among DMRs in discovery cohort 1. A Volcano plot of DMRs from patients with breast cancer (N = 3) versus healthy individuals (N = 3). Significantly hypermethylated windows were highlighted in red dots with padj < 0.05, log2Foldchange > 1, significantly hypomethylated windows were highlighted in blue dots with padj < 0.05 and log2FoldChange < − 1. B Heatmap of the 2,211 DMRs identified in plasma cfDNA from patients with breast cancer and healthy individuals. C Short fragments ratio of cfDNA in hypermethylated (H1: 0.0940; H2: 0.00994; H3: 0.0967; P1: 0.1089; P2: 0.1213; P3: 0.1322) and hypomethylated (H1: 0.1344; H2:0.1477; H3: 0.1167; P1: 0.1790; P2: 0.2017; P3: 0.1970) regions in patients with breast cancer and healthy individuals. D Percentage change of short fragments ratio in hypomethylated regions compared with it in hypermethylated regions for patients with breast cancer and healthy individuals. Hyper, hypermethylated genomic regions; Hypo, hypomethylated genomic regions; Healthy, healthy individuals; Breast, patients with breast cancer; * represents p value < 0.05