Fig. 5

The relationship between the chromatin accessibility and m6A level. A Chromatin accessibility of genes with(red) and without(blue) m6A modification. * represents p-value < 0.05, Wilcoxon test. B Comparison of m6A levels in genes with varying chromatin accessibility. Genes were classified into three categories based on their chromatin accessibility: the top 10% of genes with high accessibility, the bottom 10% of genes with low accessibility, and the remaining genes with medium accessibility. * represents p-value < 0.05, Wilcoxon test. C Changes in m6A level and chromatin accessibility during differentiation, with colors indicating 5 gene categories (group 1 to group 5) based on their distinct patterns of changes. D GO enrichment analysis results for gene categories from Fig. 5C. E Relationship between the correlation of m6A levels and accessibility, and m6A level changes at various differentiation stages. Genes were ranked according to the PCC of their m6A levels with chromatin accessibility, and every 5% of genes were grouped without top 5% and bottom 5%. PCC: Pearson correlation coefficient. F Overlap of genes with only m6A change across different differentiation stages