Fig. 1

Experimental setup: Upper left: Hepatocytes were isolated from female mice (aged 9 weeks) fed a control diet (Co, 12% kcal from fat) or a Lieber-DeCarli (LDC) diet (4% ethanol and 40.9% kcal from fat) for 6 weeks (n = 2 per group). Thereafter, RNA-seq, ChIP-seq, DNase1-seq, and RRBS were performed for epigenomic analysis. Lower left: Hepatocytes were isolated from young (10 weeks) or mid-aged (40 weeks) mice were fed a control diet and underwent RRBS analyses (n = 4). Top right: Validation was done in liver tissues from young (10 weeks, n = 4), mid-aged (40 weeks, n = 7), and aged (85 weeks, n = 4) female mice fed a control diet by SNuPE and MiSeq. Figure created with BioRender.com