Fig. 2
From: Live-cell imaging of chromatin contacts opens a new window into chromatin dynamics
Example image of ANCHOR and dCas9 labeling A: Image of a live HCT116 cell with two labeled loci, the D4Z4 repeats and a neighboring TAD boundary that are roughly 15 Mbp apart on chromosome X. The D4Z4 repeats were labeled with and a repeat-specific gRNA and dCas9-eGFP(green), the neighboring TAD boundary was visualized with an ANCH3 insertion in combination with the OR3-HaloTag protein and JF-549 dye (red). The image was taken on a SP5 Leica confocal microscope. B: Image of a live mouse embryonic stem cell with a locus on chromosome 4 visualized by an ANCH3 insertion and OR3-Halo/JF-646. The image was taken on a SpinSR SoRa Olympus spinning disk microscope. Note that the locus was already replicated and the two sister chromatids are visible