Fig. 8

NHEJ independent recruitment of ac4C RNA to microirradiated chromatin. A 3D-projection of 60 confocal sections shows the high density of ac4C RNA (red) in microirradiated chromatin and in the proximity of nucleoli (visualized by GFP-tagged UBF1/2) [36]). Analysis was performed using immortalized wild type iMEFs. The 3D projection and density of ac4C RNA and GFP-UBFs were studied using LEICA AF software. B ac4C RNA was recruited to microirradiated chromatin in 53BP1 double null (dn) MEFs, in a similar density as shown in panel (A) for the wt MEFs. The scale bars show 5 µm. C ac4C RNA significantly accumulated at microirradiated chromatin of RIF1 (wt) and RIF1 double null (dn) HCT116 cells. The scale bar shows 2 µm