Fig. 6

Recruitment of ac4C RNA to UVA-damaged chromatin is PARP-dependent. A UVA irradiated MEFs as a whole-cell population, and B microirradiated MEFs by the use of a 355-nm UVA laser. Local laser microirradiation showed that ac4C RNA did not accumulate to DNA lesions when the cells were treated with the PARP inhibitor. DNA damage was detected by antibodies against ATM and γH2AX. Scale bars are 5 µm. C anti-ac4C dot blot a (#ab252215, Abcam) in samples irradiated by UVC, treated by PARP inhibitor and treated with both PARP inhibitor and UVC irradiation. Panel b shows the quantification of the dot blot from (a)