Fig. 5

Alignment of interphase genome A/B compartments along chicken chromosome 14 with chromomeric pattern of the corresponding lampbrush chromosome. a—Distribution of A (red) and B (dark blue) compartments along the chicken chromosome 14 (GGA14) in embryonic fibroblasts viewed by Integrative Genomics Viewer (IGV) (according to [15]); b—cytological map of chicken lampbrush chromosome 14 depicting DAPI-staining pattern of chromomeres and relative contour length of lateral loops, black circles—dense chromomeres brightly stained with DAPI (according to [54]). Dotted lines in a, b connect the genomic positions of the BAC-clones (Additional file 1: Table S1) with their positions on the cytological map; c, d—DNA + RNA-FISH with BAC-clone based DNA-probes (Additional file 1: Table S2) covering the genomic regions 1–2 Mb cʹ and 10–13 Mb dʹ on chicken lampbrush chromosome 14, dotted lines from b to c and d indicate positions of the regions on the cytological map; cʹ, dʹ—the positions of the mapped BAC-clones relative to the somatic A/B compartments; cʹʹ, dʹʹ—schematic drawing of the FISH-mapping of the selected genomic regions (c, d) on lampbrush chromatin domains; colors correspond to the colors of the labeled DNA-probes on the FISH images. Scale bar: 10 μm