Fig. 4

Alignment of interphase genome A/B compartments along chicken chromosome 7 with chromomeric pattern of the corresponding lampbrush chromosome. a—Distribution of A (red) and B (dark blue) compartments along the chicken chromosome 7 (GGA7) in embryonic fibroblasts viewed by Integrative Genomics Viewer (IGV) (according to [15]); b—cytological map of chicken lampbrush chromosome 7 depicting DAPI-staining pattern of chromomeres and relative contour length of lateral loops, black circles–dense chromomeres brightly stained with DAPI. Dotted lines in a, b connect the genomic positions of the BAC-clones (Additional file 1: Table S1) with their positions on the cytological map; c—lampbrush chromosome 7 stained with DAPI, pixel intensities displayed with multicolored ImageJ look-up table, numbered line in a and c indicates position of chromomere cluster brightly stained with DAPI; d, dʹ–DNA+RNA-FISH with BAC-clone based DNA-probes (Additional file 1: Table S2) covering the genomic region 12–14 Mb on chicken lampbrush chromosome 7; dotted lines from c to d, d′ indicate chromosomal positions of the region on microphotographs; dʹʹ—schematic drawing of the FISH-mapping of the selected genomic region (d, dʹ) on lampbrush chromatin domains; colors correspond to the colors of the labeled DNA-probes on the FISH images. e—the positions of the mapped BAC-clones relative to the somatic A/B compartments. Scale bar: c—20 μm, d, dʹ—10 μm.