Fig. 3

Alignment of interphase genome A/B compartments along chicken chromosome 4 with chromomeric pattern of the corresponding lampbrush chromosome. a—Distribution of A (red) and B (dark blue) compartments along the chicken chromosome 4 (GGA4) in embryonic fibroblasts viewed by Integrative Genomics Viewer (IGV) (according to [15]); b—cytological map of chicken lampbrush chromosome 4 depicting DAPI-staining pattern of chromomeres and relative contour length of lateral loops, black circles—dense chromomeres brightly stained with DAPI (according to [53]). Dotted lines in a, b connect the genomic positions of the selected BAC-clones (Additional file 1: Table S1) with their positions on the cytological map; c—lampbrush chromosome 4 stained with DAPI, pixel intensities displayed with multicolored ImageJ look-up table, numbered lines in a and c indicate positions of chromomere clusters brightly stained with DAPI; d—DNA+RNA-FISH with BAC-clone based DNA-probes (Additional file 1: Table S2) covering the genomic region 34–37 Mb (d) on chicken lampbrush chromosome 4; dotted lines from c to d indicate chromosomal position of the region on microphotographs; dʹ—the positions of the mapped BAC-clones relative to the somatic A/B compartments; dʹ—schematic drawing of the FISH-mapping of the selected genomic region on lampbrush chromatin domains; colors correspond to the colors of the labeled DNA-probes on the FISH image. Scale bar: c—20 μm, d—10 μm.