Fig. 2

Alignment of interphase genome A/B compartments along chicken chromosome 2 with chromomeric pattern of the corresponding lampbrush chromosome. a—Distribution of A (red) and B (dark blue) compartments along the chicken chromosome 2 (GGA2) in embryonic fibroblasts viewed by Integrative Genomics Viewer (IGV) (according to [15]); b—cytological map of chicken lampbrush chromosome 2 depicting DAPI-staining pattern of chromomeres and relative contour length of lateral loops, black circles—dense chromomeres brightly stained with DAPI (according to [53, 54]). Dotted lines in a, b connect the genomic positions of the selected BAC-clones and chromosomal marker structures (Additional file 1: Table S1) with their positions on the cytological map; c—lampbrush chromosome 2 stained with DAPI, pixel intensities displayed with multicolored ImageJ look-up table, numbered lines in a and c indicate positions of chromomere clusters brightly stained with DAPI; d, e—DNA+RNA-FISH with BAC-clone based DNA-probes (Additional file 1: Table S2) covering the genomic regions 39–40 Mb (d), 128–135 Mb (e) on chicken lampbrush chromosome 2; dotted lines from c to d and e indicate chromosomal positions of the regions on microphotographs; dʹʹ, eʹ—the positions of the mapped BAC-clones relative to the somatic A/B compartments; d, eʹʹ—schematic drawings of the FISH-mapping of the selected genomic regions on lampbrush chromatin domains; colors correspond to the colors of the labeled DNA-probes on the FISH images. Scale bar: c—20 μm, d, e—10 μm.