Fig. 3

Custom filtering of putative false-positive mutations. The top 20 mutated genes are show before (A) and after (B) applying the custom filters. This shows that without this filtering step, a number of genes score positive in 100% of the patients, including gene families containing variable number of tandem repeats (e.g., the MUC gene family). After applying a threshold (maximum of 4 variants per gene in a single patient, in a maximum of 20% of the patients) and selecting the option to retain genes present in the COSMIC database, the resulting mutated gene list is highly enriched in known oncogenic drivers and previously reported AEL-mutated genes