Fig. 2

Gene expression knockdown of Dnmt1 leads to increasing differential gene expression as testes initiate and progress through gamete formation after treatment. Here, samples are visualized with a Principal Component Analysis visualization. Each sample is represented by a smaller symbol corresponding to its treatment, while the larger symbol represents the centroid of the treatment. Differential gene expression is presented with a Volcano plot; each dot representing a gene. A Samples 7-day post-treatment are highly overlapping and not differentiated by many gene expression differences. B Very few genes were statistically significantly differentially expressed (represented as red dots). C Samples 14-day post-treatment are highly non-overlapping and differentiated by many gene expression differences. D Many genes are statistically significantly differentially expressed at 14 days (~ 45% of expressed genes are different between the treatments)