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Table 1 Quality parameters for sperm isolated from caput, corpus, cauda epididymis tracts

From: CpG DNA methylation changes during epididymal sperm maturation in bulls

Parameter

Epididymal tracts

P-value

 

Caput

Corpus

Cauda

 

MOT_TOT (%)

61.76 ± 6.03 

70.31 ± 6.03 

74.25 ± 6.03 

NS

STAT (%)

38.23 ± 6.03 

29.68 ± 6.03 

25.75 ± 6.03 

NS

PROG (%)

15.08 ± 2.61a 

19.50 ± 2.61a 

39.93 ± 2.61 b 

 < 0.001

NOPROG (%)

46.61 ± 5.05 

50.86 ± 5.05 

34.30 ± 5.05 

NS

VCL (µm/s)

89.51 ± 4.27 a 

97.59 ± 4.27 a 

120.05 ± 4.27 b 

 < 0.0001

VSL (µm/s)

18.48 ± 4.02 a 

22.49 ± 4.02 a 

59.81 ± 4.02 b 

 < 0.001

VAP (µm/s)

33.70 ± 3.91 a 

38.65 ± 3.91 a 

73.15 ± 3.91 b 

 < 0.001

LIN (%)

20.69 ± 2.64 a 

22.89 ± 2.64 a 

48.95 ± 2.64 a 

 < 0.0001

STR (%)

55.41 ± 2.48 a 

57.33 ± 2.48 a 

80.15 ± 2.48 b 

 < 0.0001

WOB (%)

37.61 ± 2.62 a 

39.52 ± 2.62 a 

60.34 ± 2.62 b 

 < 0.0001

ALH (µm)

3.52 ± 0.16 a 

3.93 ± 0.16 a 

3.97 ± 0.16 a 

NS

BCF (Hz)

4.78 ± 0.36 a 

5.32 ± 0.36 a 

7.09 ± 0.36 b 

 < 0.0001

V (%)

62.02 ± 3.13 a 

53.60 ± 3.13 ab 

65.87 ± 3.13 ac 

 < 0.05

D (%)

37.98 ± 3.15 a 

46.70 ± 3.15 b 

34.16 ± 3.15 a 

 < 0.05

VI (%)

61.00 ± 3.16 a 

51.13 ± 3.16 b 

64.13 ± 3.16 a 

 < 0.05

VR (%)

1.01 ± 0.32 a 

2.47 ± 0.32 b 

1.71 ± 0.32 a 

 < 0.05

DI (%)

33.07 ± 2.58 a 

37.91 ± 2.58 ab 

27.10 ± 2.58 ac 

 < 0.05

DR (%)

4.90 ± 0.79 a 

8.78 ± 0.79 b 

7.06 ± 0.79 a 

 < 0.05

Alpha-T

0.41 ± 0.001a 

0.40 ± 0.001ab 

0.40 ± 0.002bc 

 < 0.05

ATSD

0.012 ± 0.001 

0.016 ± 0.001 

0.0153 ± 0.001 

NS

DFI (%)

1.003 ± 0.236 

1.638 ± 0.236 

1.567 ± 0.253 

NS

HG (%)

1.527 ± 0.435a 

2.115 ± 0.435ab 

0.699 ± 0.466ac 

NS

HDS

3.346 ± 0.0449a 

3.531 ± 0.0449b 

3.307 ± 0.0481a 

 < 0.001

  1. MOT TOT total motility, STAT immotile cells, PROG cells progressive motility, VSL straight-line velocity, VCL curvilinear velocity, VAP average path velocity, LIN linear coefficient, STR straightness coefficient, WOB wobble coefficient, ALH amplitude of lateral head displacement, BCF beat cross-frequency, V viable sperm, D death sperm, VI viable with intact acrosome, VR viable with disrupted acrosome, DI death with intact acrosome, DR death with disrupted acrosome, Alpha-T red/(red + green) fluorescence intensity, ATSD Alpha-T standard deviation, DFI fragmented DNA sperm, HG high green fluorescence sperm, HDS: proportion of immature spermatozoa lacking the normal exchange of histone for protamine-complexed DNA. a,b,c, values within a row with different superscript letters differ significantly at P < 0.05
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Epigenetics & Chromatin

ISSN: 1756-8935