Table 4 Secondary antibodies
Antibody | Fluorophore | Use dilution | Reference | Supplier |
|---|---|---|---|---|
CyTM2-goat anti-rabbit | Cy2 (green) | IF | 111-225-144 | Jackson Immunoresearch |
1:1000 | ||||
CyTM3-goat anti-mouse | Cy3 (red) | IF | 15-165-146 | Jackson Immunoresearch |
1:1000 | ||||
CyTM5-goat anti-mouse | Cy5 (far red) | IF | 115-175-146 | Jackson Immunoresearch |
1:1000 | ||||
Goat anti-mouse IgG | DyLight 680 (red) | WB 1:10,000 | 35,518 | Thermo Fisher Scientific |
Goat anti-rabbit IgG | DyLight 800 (green) | WB 1:10,000 | 35,571 | Thermo Fisher Scientific |