Table 3 Primary antibodies
Antibody | Type | Dilution (WB/IF) | Clon, reference | Supplier |
|---|---|---|---|---|
H3K4me3 | Rabbit polyclonal | 1:800 | 9727 | Cell signaling |
1:1000 | ||||
H3K9ac | Rabbit polyclonal | 1:2000 | 07-352 | Millipore |
1:1000 | ||||
H3K9me3 | Rabbit polyclonal | 1:800 | 07-442 | Millipore |
1:800 | ||||
H3K27ac | Rabbit polyclonal | 1:1000 | 07-360 | Millipore |
1:1000 | ||||
H3K27me3 | Rabbit polyclonal | 1:800 | 07-449 | Millipore |
1:800 | ||||
Histone H3 | Rabbit polyclonal | 1:1000 | 9715 | Cell signaling |
– | ||||
VRK1 | Mouse monoclonal | 1:1000 | 1F6 | [83] |
– | ||||
VRK1 | Rabbit polyclonal | 1:1000 | VC | [83] |
– | ||||
HA.11 tag | Mouse monoclonal | 1:1000 | 901514, 16B12 | BioLegend |
– | ||||
HA tag | Rabbit polyclonal | 1:1000 | H6908 | Sigma-Aldrich |
– | ||||
Myc tag | Mouse monoclonal | 1:1000 | 4A | Millipore |
– | 05-724 | |||
Myc tag | Rabbit polyclonal | 1:1000 | 06-549 | Millipore |
– | ||||
V5 tag | Mouse monoclonal | 1:1000 | V5-10 | Sigma-Aldrich |
– | V8012 | |||
V5 tag | Rabbit polyclonal | 1:1000 | V8137 | Sigma-Aldrich |
– | ||||
β-actin | Mouse monoclonal | 1:2000 | AC15 | Sigma-Aldrich |
– | A5441 | |||
Flag tag | Mouse monoclonal | 1:1000 | M5 | Sigma-Aldrich |
– | F4042 | |||
Flag tag | Rabbit polyclonal | 1:1000 | F7425/ab1162 | Sigma-Aldrich/Abcam |
– |