Table 2 Plasmids
Protein or tag | Plasmid | Tag | Expression | Supplier, reference |
|---|---|---|---|---|
EZH2 | pCMV6 | Myc-DDK | Mammalian | Origene, #RC202054 |
Flag-Ø | pCEFL | Flag | Mammalian | Gift from JS Gutkind (NIH) |
GST-Ø | pGEX-2TK | GST | Bacterial | GE Healthcare GE28-9546-53 |
HA-Ø | pCEFL | HA | Mammalian | Gift from JS Gutkind (NIH) |
HDAC1 | pcDNA3.1+ | Flag | Mammalian | Addgene, #13820 |
KDM1A (LSD1) | pCMV6 | Myc-DDK | Mammalian | Origene, #RC208480 |
KDM3A (JMJD1A) | pLenti | V5-Myc | Mammalian | Addgene, #82331 |
KDM4A (JMJD2A) | pCMV | HA | Mammalian | Addgene, #24180 |
PCAF | pCl | Flag | Mammalian | Nakatani [81] |
SETDB1 | pIRES2-EGFP | Flag | Mammalian | Rodriguez Paredes [82] |
VRK1 | pCEFL | HA | Mammalian | Valbuena et al. [83] |
VRK1 | pcDNA3.1 | Myc | Mammalian | Valbuena et al. [83] |