Fig. 4
From: Caf1 regulates the histone methyltransferase activity of Ash1 by sensing unmodified histone H3
Caf1 in AMC complex senses unmodified histone H3 on nucleosome array. A–C HMT assay using G5E4 nucleosome array and AMC complex (70, 100 and 140 nM) (top panels). The relative activity of AMC complexes on unmodified nucleosome array is shown in blue, and H3K4me3 nucleosome array in magenta (bottom panels): AMC wild-type (AMC WT), AMC with Caf1 H3 binding mutant (E235Q/D252K/E279Q) (AMC mut) and DOT1L H3K79 methyltransferase (DOT1L) (n = 3, ****p value < 0.0001, n.s. = not significant). D, E HMT assay using mononucleosome with 80 bp linker DNA (top panels) and the relative activity shown in a bar graph (n = 3, n.s. = not significant) (bottom panels)