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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: An optimized approach for multiplexing single-nuclear ATAC-seq using oligonucleotide-conjugated antibodies

Fig. 2

Key steps in NuHash library preparation. The snATAC-seq library preparation and NuHash library preparation are shown in the left and right panels, respectively. The NuHash oligonucleotide is composed of Illumina read1N (black), read2N (gray), two UMIs (yellow), and a sample-specific hash barcode (purple). The read1Ns are used for capturing by GEMs. The captured products are amplified linearly, during which the Illumina P5 adapter (blue) and 10 × barcode (green, a unique identifier for GEM) sequences are also added. Illumina P7 (orange) and sample index barcode (light gray) sequences are added to the products at the PCR amplification step. The full NuHash products contain full-length Illumina sequencing adaptors at both ends, 10 × barcode, two UMIs, NuHash barcode, and sample index barcode (bottom right)

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Epigenetics & Chromatin

ISSN: 1756-8935