Skip to main content
Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: An optimized approach for multiplexing single-nuclear ATAC-seq using oligonucleotide-conjugated antibodies

Fig. 1

NuHash sample multiplexing schematic overview. Isolated nuclei from each sample are stained with a NuHash oligonucleotide-conjugated antibody containing a unique sample barcode. The stained nuclei from different samples were pooled for snATAC-seq library preparation, followed by massively parallel sequencing. Sequencing reads were then assigned to each nucleus using a nuclei (10x) barcode, and demultiplexing of nuclei from individual samples is carried out using the NuHash reads matching the sample barcode

Back to article page

Epigenetics & Chromatin

ISSN: 1756-8935