Fig. 4
From: Multilevel interrogation of H3.3 reveals a primordial role in transcription regulation
Cac2Tt and Hir1Tt show distinct localization during growth and development in Tetrahymena. A HirTt-FZZ localizes to both MAC and MIC during vegetative growth and exclusively to the cytoplasm during starvation. HirTt-FZZ cells were mated with untagged WT cells of different mating type. During conjugation, Hir1Tt-FZZ localizes to the four meiotic products and the parental MAC after the completion of meiosis. Hir1Tt-FZZ staining persisted in the parental MAC and the selected pronucleus only. B Cac2Tt-FZZ localizes predominantly to the MIC and faintly to the MAC during vegetative growth. Cac2Tt-FZZ signal was observed exclusively in the MIC during starvation. During conjugation, Cac2Tt-FZZ staining was observed in the crescent MIC as well as in the selected pronucleus. Note: Nuclear events are depicted above the images taken for conjugating cells during various developmental stages. DAPI was used to stain the nuclei. The signal observed in both mating types at the anlagen stage is due to the mixing of cellular contents between the pairing cells. CU428, mating type VII, and B2086, mating type II are the strain numbers of the strains obtained from the Tetrahymena Stock Center, Cornell University