Fig. 4

Proteins enriched in KDM5 TurboID experiments show overlapping genomic binding profiles in Drosophila and human cells. A Venn diagram showing overlap between high confidence biotinylated proteins (red) and datasets enriched when comparing published KDM5 ChIP-seq data from whole adult flies to other Drosophila ChIP datasets (blue). A total of 27 datasets showed significant overlap using ChIP-atlas. 30 interactors had ChIP-seq datasets in the ChIP Atlas database (green). B Volcano plot showing fold enrichment and p-values of the permutation analyses between KDM5 and 27 ChIP-seq datasets of high confidence interactors. C Heat maps showing ChIP-seq genomic binding profiles of Sin3A from S2 cells and KDM5 from whole adult flies. D Genomic binding profiles of BEAF-32 ChIP-seq from S2 cells and KDM5. E Genomic binding profiles of Dref ChIP-seq from Kc167 cells and KDM5. F Genomic binding profiles of Iswi ChIP-seq from Kc167 cells and KDM5. G Genomic binding profiles of fs(1)h ChIP-seq from Kc167 cells and KDM5. H Representative genome browser image showing binding of KDM5, Sin3A, BEAF-32, Dref, fs(1)h, and Iswi. I Venn diagram showing overlap between human orthologs of Drosophila KDM5 high confidence interactors (HCI) and their enrichment when comparing published KDM5B ChIP-seq data to breast cancer ChIP-seq datasets using ChIP-Atlas. 26 datasets significantly overlapped. J Volcano plot showing fold enrichment and p-values of the permutation analyses between KDM5B and 26 ChIP-seq datasets of human ortholog-converted high confidence interactors. K Genomic binding profiles of KDM5B, SIN3A and BRD4 showing similar genome-wide binding. Binding is shown relative to KDM5B due to the much larger number of SIN3A and BRD4 binding sites in the genome compared to KDM5B. L Genomic binding profiles of MED1 ChIP from MCF-7 cells and KDM5B showing similar localization. M Genomic binding profiles of SUMO2 (ortholog of Drosophila Sumo) ChIP from MCF-7 cells and KDM5B showing similar localization. N Representative genome browser image showing binding of KDM5B, SIN3A, MED1, BRD4 and SUMO2