Fig. 3

TurboID-tagged KDM5 biotinylates proteins involved in several aspects of gene expression regulation. A STRING analyses of nuclear proteins that were significantly biotinylated by NT-KDM5 and CT-KDM5. Grey lines indicate known physical interactions between proteins. Darker lines indicate a higher confidence of interaction. Cytoscape was used to manually cluster annotated proteins based on their STRING Cluster Enrichment and known functions based on published literature. Unconnected nodes and proteins with unclear links to known complexes are not shown. B Gene Ontology Biological Process (GO-BP) analyses of the 87 high confidence KDM5 interacting proteins. C Structure of the human pre-initiation complex (PDB accession: 7ENA) showing proteins identified as high confidence interactors in our TurboID data in red bubbles. DNA (yellow), TBP (pink bubbles) and RNA polymerase II (cyan bubbles) are also shown