Fig. 2
From: Proximity labeling reveals a new in vivo network of interactors for the histone demethylase KDM5
Identification of high confidence KDM5 interactors through TurboID. A Volcano plot showing data comparing biotinylated proteins enriched by NT-KDM5 to control. B Volcano plot showing data comparing CT-KDM5 to control. C Volcano plot showing data comparing NT-KDM5 to TurboID. D Volcano plot showing data comparing CT-KDM5 to TurboID. E Volcano plot showing data comparing NT-KDM5 to dCas9:TurboID. F Volcano plot showing data comparing CT-KDM5 to dCas9:TurboID. G Filtering workflow for identification of high confidence KDM5 interactors by combining data from experiments 1 and 2. H Summary of known Drosophila KDM5 interactors, whether the interaction is conserved in mammals (mouse or human) and the identification of these proteins in experiment 1 and experiment 2 compared to control and dCas9:TurboID. Three interactors identified in mammalian cells but not previously in Drosophila are also included (Caf1-55, Mi-2 and Zmynd8). I High confidence interactors (HCI) based on their identification in experiment 1 (compared to control) and experiment 2 (compared to control and dCas9:TurboID). Dark red box indicates enrichment of p < 0.05, pink indicates p < 0.1. For all volcano plots shown, known interactors are indicated with text. Red dots indicate significantly enriched proteins (p < 0.05) and the dotted line on Y-axis indicates p = 0.05