Fig. 6
From: The SAGA histone acetyltransferase module targets SMC5/6 to specific genes
Hypothetical model of SAGA-mediated loading of SMC5/6 complex. The SAGA histone acetyltransferase (HAT; red) module binds several SMC5/6 subunits (top panel; Nse2: black, Smc5 arm: blue, and Nse3: green; model based on Ref. [15]). SAGA complex (violet; model based on Ref. [80]) binds promoter regions and targets rod-shaped SMC5/6 complex to specific sites (middle panel). Then, Gcn5-mediated acetylation of the H3 histone tails (dark green) helps to open chromatin (at least in a subset of cases) and expose free DNA to SMC5/6 (bottom panel). Open chromatin stimulates Nse3-mediated binding to free DNA [22] accompanied by SMC5/6 conformational change. Such ring-shaped conformation of SMC5/6 (model based on [17]) completely or partially dissociates from the SAGA complex (Additional file 1: Fig. S4)