Fig. 4

SMC5/6 distribution is dependent on the SAGA HAT module. A The pie chart shows the distribution of the Nse4-FLAG peak areas in the different genome regions in the WT fission yeast cells. Most SMC5/6 is localized to the repetitive regions (like rDNA and centromeres), with the highest occupancy of the rDNA repeats. A significant portion of the Nse4-FLAG is localized within the intergenic regions or genes. B The heatmap diagrams compare the occupancy of Nse4-FLAG peaks in the WT, Δgcn5, Δada2 and Δubp8 mutant cells (as identified in the WT). The top part shows peaks enhanced in Δgcn5 and Δada2 (enhanced), whilst the bottom part clusters peaks reduced in the SAGA HAT module deficient cells (reduced). Peaks in the rDNA repeats are shown separately as these chromosome regions are not fully assembled and annotated in the S. pombe reference genome and exert a different range of coverage values (rDNA). The Nse4-FLAG peaks (normalized to median 760 bp width) and their surrounding regions (200 bp upstream and 200 bp downstream) are shown. C The pie charts show the distribution of the enhanced (top) and reduced (bottom) Nse4-FLAG peak areas in Δgcn5. The SMC5/6 accumulation is mainly enhanced at the repetitive loci. The SMC5/6 localization is primarily reduced in gene regions. D The box plot graph compares the Nse4-FLAG occupancy in WT and Δgcn5 cells. The paired two-sided Wilcoxon statistical test was used: ns, non-significant; ***p < 0.001