Fig. 3
From: The SAGA histone acetyltransferase module targets SMC5/6 to specific genes
SMC5/6 and SAGA are required for efficient DNA repair. A Sensitivity of the SMC5/6 and SAGA mutants to genotoxins. Ten-fold serial dilutions of the yeast strains were plated onto YES media containing indicated concentrations of the hydroxyurea (HU) or methyl methane sulfonate (MMS). The double mutants were more sensitive than their respective single mutant counterparts, suggesting the non-redundant functions of SMC5/6 and SAGA in DNA repair. At least three independent drop tests were carried out, and only one of them is displayed as representative. B Live-cell microscopy of endogenous Nse4-GFP upon HU and MMS treatment, respectively. The Nse4-GFP foci were present in the WT and Δgcn5 cells but largely absent in Δbrc1 cells. C Quantification of the data in B suggests that the localization of SMC5/6 to the DNA-damage foci is independent of SAGA