Fig. 1

Experimental model and clustering of samples based on DNA methylation patterns using 100,000 randomly sampled CpGs. A Schematic illustration of lens epithelial and fiber cells compartments. B Violin plots showing distribution of CpG methylation scores across genomic features for all four groups lens samples and representative non-lens cells, including NPC and ES cells. Bottom panel: schematic of genomic feature annotations. C Left: PCA showing distinction in methylation patterns of sampled CpGs among lens samples and representative non-lens cells. Right: magnification of inset from left panel showing the three developmental path segments, path “epithelial” differentiation, Epi(dif), path “epithelial to fiber cell” differentiation at E14.5, EpiFiber(dif), and path “fiber cell” differentiation, Fiber(dif), respectively. The color codes of each sample are shown on the right