Table 2 Sequence and vendor details for synthetic histone peptides
From: De novo methylation of histone H3K23 by the methyltransferases EHMT1/GLP and EHMT2/G9a
Peptide name | Sequence | Vendor/catalog # | Experiment |
|---|---|---|---|
H3K9me1 | ARTKQTARK(me1)STGGKAPRKQLK-biotin | EpiCypher; 12–0010 | ELISA/antibody validation |
H3K9me2 | ARTKQTARK(me2)STGGKAPRKQLK-biotin | Active Motif; 81046 | ELISA/antibody validation |
H3K9me3 | ARTKQTARK(me3)STGGKAPRKQLK-biotin | Active Motif; 81047 | ELISA/antibody validation |
H3K18me1 | ARTKQTARKSTGGKAPRK(me1)QLK-biotin | Epicypher; 12–0013 | ELISA/antibody validation |
H3K18me2 | ARTKQTARKSTGGKAPRK(me2)QLK-biotin | Epicypher; 12–0014 | ELISA/antibody validation |
H3K18me3 | ARTKQTARKSTGGKAPRK(me3)QLK-biotin | Epicypher; 12–0015 | ELISA/antibody validation |
H3K23me1 | KQLATK(me1)AARKSAPATGGVYK-biotin | Custom made/Biomatik | ELISA/antibody validation |
H3K23me2 | KQLATK(me2)AARKSAPATGGVYK-biotin | Custom made/Biomatik | ELISA/antibody validation |
H3K23me3 | KQLATK(me3)AARKSAPATGGVYK-biotin | Custom made/Biomatik | ELISA/antibody validation |
H3K27me1 | ATKAARK(me1)SAPSTGGVKKPHRYRPGGGK-biotin | Active Motif; 81050 | ELISA/antibody validation |
H3K27me2 | ATKAARK(me2)SAPSTGGVKKPHRYRPGGGK-biotin | Active Motif; 81051 | ELISA/antibody validation |
H3K27me3 | ATKAARK(me3)SAPSTGGVKKPHRYRPGGGK-biotin | Active Motif; 81052 | ELISA/antibody validation |
H3K9M | ARTKQTARMSTGGKAPRKQLK-biotin | Made in house | Peptide pulldown |
H3K23M | KQLASMAARKSAPATGGIK-biotin | Made in house | Peptide pulldown |
H3 unmodified (1–25) | ARTKQTARKSTGGKAPRKQLATKAA-biotin | Epicypher; 12–0107 | ELISA, peptide pulldown |
H3 unmodified (18–36) | KQLATKAARKSAPATGGVYK-biotin | Custom made/Biomatik | ELISA, peptide pulldown |