Fig. 4

UV-exposed cells experience persistent increases in cell size and changes in cell wall composition. Log-phase suspensions were exposed to UV at 50 J/m², or mock exposed, followed by incubation, as described in Fig. 1. Samples were collected from exposed cultures and unexposed controls at indicated timepoints and stained with calcofluor white. Cells were visualized by fluorescent microscopy and analyzed for cross-sectional cell area and fluorescent intensity. A Representative images from unexposed (−UV) and exposed (+ UV) cultures, following a 4 h incubation (400X magnification). B Cross-sectional cell area, reported as means ± 1 SE, normalized relative to the corresponding −UV cell size for each condition (*p < 0.01). C Calcofluor white (CFW) staining intensity, reported as means ± 1 SE, normalized relative to the corresponding −UV CFW staining intensity for each condition (*p < 0.01)