Fig. 4

Open chromatin profile changes in life forms. A IGV snapshot of contig PRFA01000005 showing FAIRE-seq profile distribution in epimastigote (red), metacyclic (blue), and control samples (gray). PTUs are shown in blue tracks with the transcription direction indicated by arrows. Genes from disruptive (red), core (green), and those from both (orange) compartments are depicted. tDNAs are shown in dark blue. B Comparison of core and disruptive compartments using RPGC counts. *** Wilcoxon–Mann–Whitney test with p-value = 0.001. C Scatter plots of E/MT RPGC levels on each indicated feature. Median values are written between parentheses. One-way ANOVA with Dunnett’s correction. D Hierarchical cluster analysis of the distribution of the RPGC log2 ratio (E/MTs) in PTUs considering (top) or not (below) 1 kb upstream or downstream. The number of members in each cluster is depicted next to the graph. The first base of the feature is represented by Start, while the last base is End. MT-metacyclic