Fig. 4

Colocalisation and co-immunoprecipitation of TET1 and PARP-1 proteins in NIH3T3 cells. a Confocal imaging of PARP-1 (green) and TET1 (red) stained by fluorescent secondary antibodies. b Colocalisation is evidenced by the merged confocal image and the image where sites of colocalisation (points where both PARP-1 and TET1 signals are detected) are highlighted in white. c Quantification of colocalisation rate from three replicate cover-slips analysed. For each cover-slip, 10 images were analysed and the colocalisation rate is shown as mean ± SD (n = 3). d Example of colocalisation in one enlarged nucleus (marked by □ in panels a and b). The intensity of green and red signals is measured along the green line (ROI1). n‐number of independent experiments, a.u.—arbitrary units, IP—immunoprecipitation, Ab—antibody, WB—western blot