Fig. 5

Analysis of differentially expressed genes between Sus scrofa ICSI- and mICSI-derived blastocysts. A Functional annotations of DEGs in the biological process subcategory cellular process (GO:0009987). B Heatmap displaying mtDNA-encoded genes in Sus scrofa ICSI- and mICSI-derived blastocysts. Rows and columns show individual genes and RNAseq samples, respectively, ordered by position in the Sus scrofa mtDNA sequence. Each tile in the main matrix represents the levels of expression of a single gene in a single RNAseq data set. Colour of tile indicates levels of expression, and a scale is presented on the right. The DNA strand of encoded genes, biotype of genes, and start position in mtDNA are also presented in colour tiles on the left. C and D Analysis of signalling pathways for differentially expressed genes using IPA. Identified signalling network 1 (C) and network 2 (D) are shown. Types of molecules are drawn in different shapes and genes that are up- or down-regulated in mICSI-derived blastocysts are indicated in red and green, respectively