Fig. 4

Asf1a regulates H3K56 acetylation, while Asf1b is involved in cell proliferation in early embryos. A Immunofluorescence detection of H3K56ac (green) in GV-stage oocytes and pre-implantation embryos. 13–45 embryos were analyzed in each group. DNA was stained with DAPI (blue). Scale bar, 20 µm. B Co-staining of Asf1a (green) and H3K56ac (red) in 4-cell, morula and blastocyst that were developed from Control-MO or Asf1a-MO microinjected zygotes. C, D Quantification of the fluorescence intensities for Asf1a and H3K56ac in blastocyst (Control-MO, n = 41; Asf1a-MO, n = 41). E Immunofluorescence detection of H3K56ac (red) in 4-cell, morula and blastocyst that was developed from Control-MO or Asf1b-MO microinjected zygotes. F Quantification of H3K56ac fluorescence intensity in blastocyst (Control-MO, n = 44; Asf1b-MO, n = 44). G Immunofluorescence detection of Oct4 in blastocyst that was developed from Control-MO or Asf1a-MO microinjected zygotes. H Quantification of Oct4 fluorescence intensity in blastocyst (Control-MO, n = 84; Asf1a-MO, n = 89). I, K Immunofluorescence detection of PCNA (green) in 4-cell, morula and blastocyst stage embryos that was developed from Asf1a-MO or Asf1b-MO microinjected zygotes. J Quantification of PCNA fluorescence intensity in morula (Control-MO, n = 60; Asf1a-MO, n = 60). L Quantification of PCNA fluorescence intensity in morula. (Control-MO, n = 36; Asf1b-MO, n = 36). All quantification data were presented as mean ± SEM, and analyzed using Student’s t-test, ***P < 0.001. DNA was stained with DAPI, scale bar 20 µm