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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: One-pot universal NicE-seq: all enzymatic downstream processing of 4% formaldehyde crosslinked cells for chromatin accessibility genomics

Fig. 3

Sonication free enzymatic condition for accessible chromatin from 25 to 5 K HCT116 cells. A Pearson correlation of accessible chromatin peak read densities between one-pot UniNicE-seq 5000, 1000, 500, 100 and 25 cells. B Comparison of FRiP scores of one-pot UniNicE-seq 5000, 1000, 500, 100 and 25 cells. C Upset plot showing common and unique accessible region peaks between one-pot UniNicE-seq 5000, 1000, 500, 100 and 25 cells. D Genome-wide metagene plot of TSS (top panel) and enhancer elements (bottom panel) with ± 2 Kb of flanking region of one-pot UniNicE-seq 5000, 1000, 500, 100 and 25 cells. Enhancer start (ES) and enhancer end (EE) site are indicated at the bottom. E Peak annotation of one-pot UniNicE-seq 5000, 1000, 500, 100 and 25 cells libraries. F Representative IGV genomic tracks of accessible chromatin of one-pot UniNicE-seq 5000, 1000, 500, 100 and 25 cells

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Epigenetics & Chromatin

ISSN: 1756-8935