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Table 1 Primer sequences used for RT-qPCR analysis

From: Ethionine-mediated reduction of S-adenosylmethionine is responsible for the neural tube defects in the developing mouse embryo-mediated m6A modification and is involved in neural tube defects via modulating Wnt/β-catenin signaling pathway

Symbol

Forward primer (5′–3′)

Reverse primer (5′–3′)

Mettl3

CGCTGCCTCCGATGTTGATCTG

ACTGACCTTCTTGCTCTGCTGTTC

Mettl14

ACACCCCGACCTGACTTGACTG

CCCACTCCTGCCACTCCTCTG

Kiaa1429

CCCAGCCACAGCCGACTTTG TC

GAGGAGGTCCAGGAGGAGTTCTTG

Alkbh5

GGTGGTGCGTGCCTCTAATTCC

GCCTCAAACCTGCGACACTCC

Fto

GTCCGTCCCAGCAGCACTTTC

ACAGCACAGCAATACAAGCAGACC

Rbm15

TCTCCTGGCTGTACCTGGAAGTTC

TCTGAGTAGAGGCGGCAGTGTC

Zc3h13

TGTGCGAGATGTGCGTGATGTC

CCATAACTGCGGGCTTCTTCCTG

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Epigenetics & Chromatin

ISSN: 1756-8935