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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Ethionine-mediated reduction of S-adenosylmethionine is responsible for the neural tube defects in the developing mouse embryo-mediated m6A modification and is involved in neural tube defects via modulating Wnt/β-catenin signaling pathway

Fig. 2

Ethionine inhibited cell proliferation in E10.5 embryos and HT-22 cells. A PCNA-positive cell (green) of E10.5 NTDs embryos and normal embryos by immunofluorescence. Cell nuclei were stained with DAPI (blue). The percentage of PCNA-positive cells in each region is shown. Scale bars, 500 μm. B, C Western blot detected the expression profiles of PCNA in embryonic tissue treated with ethionine and SAM. The bar graph shows the relative band intensity of PCNA. β-Tubulin levels were also evaluated to confirm equal loading (n = 3). ***p < 0.001 compared with WT; **p < 0.01 compared with ethionine+SAM. (D and E) Western blot analysis of the protein levels of PCNA in the HT-22 cells treated with ethionine and SAM. The bar graph shows the relative band intensity of PCNA. β-Tubulin was used as a loading control (n = 3; mean ± SEM; ***p < 0.001). F, G The cell cycle distribution in the HT-22 cells treated with ethionine and SAM was analyzed by flow cytometry. H, I The effect of SAM and ethionine on cell proliferation was detected using Cell-Light Edu Apollo DNA in vitro Kit and was quantified. Images of immunofluorescence cell staining against the EDU (shown in green), and the nuclei were counterstained using DAPI (shown in blue). Scale bar: 500 μm

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Epigenetics & Chromatin

ISSN: 1756-8935