Fig. 3
From: Revealing RCOR2 as a regulatory component of nuclear speckles
RCOR2 associates with nuclear speckles at their core region. A RCOR2 (green) and SRRM2 (red) immunolabeling in HT22 cells visualized at super-resolution confocal acquisition. Hoechst is visualized in blue color. Right panels show zoomed-in individual nuclear speckles. B Western blot analysis of RCOR2, SRRM2, SON, and SRSF7 distribution on cytosolic, nuclear and chromatin fractions of HT22 cells. GAPDH and H3 were assayed as cytosolic and chromatin markers, respectively. Western blot is representative of two independent experiments. Numbers in nuclear fractions indicate the NaCl concentration (mM) at which the soluble fraction was extracted. S: supernatant. P: pellet. C Western blot analysis of co-immunoprecipitation of RCOR2, SON and HP1α in nuclear soluble fractions enriched in RCOR2 from HT22 fractionation experiments. Ab: antibody used to immunoprecipitate RCOR2. IgG: immunoglobulin G used as a specificity control. D, E Western blot analysis of co-immunoprecipitation of RCOR2 and nuclear speckle components using HT22 (D) and N2A (E) total cell extracts as input. LSD1 was assayed as a positive control for RCOR2 interactors. Ab: Antibody used for immunoprecipitation of RCOR2. IgG: Immunoglobulin G used as a specificity control