Fig. 7

Phase-separated compartments formed from overexpression of histone deacetylase 4. SK-N-SH neuroblastoma cells were transfected with histone deacetylase 4-green fluorescent protein (HDAC4-GFP) expression vector and counterstained with Hoechst 33342 (DNA). The circles highlight condensates that form spontaneously upon overexpression of HDAC4-GFP. These are found in chromatin-poor regions of the nucleus outside of the nucleolus. The box highlights a heterochromatic region of the nucleus. Note the inverse relationship between HDAC4-GFP concentration and chromatin concentration, further highlighting that the condensates are forming outside of dense regions of chromatin. The scale bar represents 5 µm