Fig. 2

Loss of MORC3 results in upregulation of TEs. a, b Volcano plot showing log₂fold change in TE expression between WT and Morc3^MD41/MD41 (MommeD line, a), and between WT and Morc3^–/– (CRISPR line, b). TE expression of three replicates were measured and log₂fold change >|1| and p(adj)-value < 0.05 was considered significant. Reads were allowed to multimap and a published TE transcripts pipeline was used to determine differentially expressed TEs [27]. c, d Barplot showing the distribution of upregulated TEs in the MommeD line (c) and the CRISPR line (d). e Barplots comparing the length of TEs that are upregulated in the MORC3 mutant lines with the length of all expressed TEs. Mann–Whitney U test was used to test for significance (p-value = 8.8e−73 in the MommeD line, p-value = 6.8e−39 in the CRISPR line)