Fig. 5

Effect of H2A.X KO on markers of caspase-initiated chromatin condensation in HUDEP-2 cells. A Western blot analysis of H2A.X pS139, H2A.X pY142, H2B pS14, H2B K15Ac, and H4 K16Ac post-translational marks in proliferating WT (n = 3) and H2A.X KO HUDEP-2 (KO1–KO3) (n = 2) subclones during proliferation. HSC70 was used as loading control. B Western blot analysis of full-length Caspase-3 protein expression in WT (n = 3) and H2A.X KO (n = 6) HUDEP-2 cell cultures at D0, D4, and D7 of maturation. HSC70 was used as loading control. Quantification was performed using ImageJ, with signal observed in WT at D0 set as 1.0. Error bars represent ± SEM. Asterisks (*) indicate significance as follows: ***p ≤ 0.001. p-values were calculated with a two-tailed Student’s t-test