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Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: Programmed suppression of oxidative phosphorylation and mitochondrial function by gestational alcohol exposure correlate with widespread increases in H3K9me2 that do not suppress transcription

Fig. 4

Widespread increases in histone H3, lysine 9 dimethylation does not associate with increased transcriptional suppression. A We assayed cellular extracts isolated from the fetal cortex of control, EtOH-exposed unaffected, and EtOH-exposed affected offspring for the enrichment of histone H3, lysine 9 dimethylation (H3K9me2) using chromatin immunoprecipitation (ChIP) followed by qPCR analysis. Our ChIP-qPCR primers assayed H3K9me2 within an untranscribed region of chromosome 6 (Untr6) and the indicated transposable elements. B RT-qPCR analysis of transcripts encoding the three families of transposable elements assayed above. Expression levels were normalized to transcripts encoding Gapdh and Ywhaz. C Analysis of global levels of H3K9me2 using western blotting. Representative western blot, with levels of H3K9me2 normalized to total histone H3 (n = 7). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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Epigenetics & Chromatin

ISSN: 1756-8935