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Table 2 Summary of a subset of results found in this analysis

From: Evaluation of whole-genome DNA methylation sequencing library preparation protocols

Short Name

Kapa

NEB

PBAT

Swift

Low NEB

Low Swift

Sample

A

B

A

B

A

B

A

B

A

B

A

B

# Sequenced Reads (Millions)

347.5

607.9

355.6

389.9

127.9

124.1

395.8

368.2

453.7

408.6

392.7

449.8

Low Quality Bases Trimmed (R1)

2.3

1.8

1.0

1.0

0.9

0.9

0.6

0.6

1.2

1.1

1.1

0.8

Low Quality Bases Trimmed (R2)

2.0

1.7

1.1

1.0

5.9

3.4

0.7

0.7

1.2

1.2

1.0

0.8

Insert Size

208.1

228.0

285.4

297.8

299.3

300.6

222.1

225.0

291.4

290.8

217.9

223.8

Duplicate Rate

27.5

32.4

9.5

9.9

13.5

11.0

12.8

12.1

18.0

16.2

30.7

26.9

% CpGs Covered

74.0

72.3

87.5

87.6

81.8

82.6

85.6

85.2

87.6

87.6

85.5

85.3

% CpG Retention

81.6

79.0

78.5

75.6

74.0

71.1

80.3

77.5

77.8

74.8

79.4

76.9

  1. All values shown are averaged across the two technical replicates, with the exception of PBAT, which only had one technical replicate. The first four rows are taken from the raw data, while the last three rows are taken from the subsampled data, where the BAMs were downsampled to be comparable to PBAT