Fig. 2

Stage II gametocytes have unique patterns of H3K36me2&3 occupancy and enrichment associated with genes. a H3K36me2&3 occupancy (average log₂ ChIP/input binned into 50 bp regions) spanning 1.7 kb upstream and 2.5 kb downstream of transcriptional start sites (TSS) of 5206 P. falciparum genes (PlasmoDB genome annotation, v39) in pre-stage II (pre-II), stage II (stg II) and post-stage II (post-II) gametocyte populations. Data are representative of the average occupancy obtained for two independent biological repeats, except for pre-stage II gametocytes with only one. Genes were rank ordered individually in each heatmap. Summary plots of the H3K36me2&3 (turquoise and purple, respectively) occupancy in each of the gametocyte stages with ribbons representing ± SE. The light grey shadowed region indicates regions of increased occupancy in the stage II gametocytes. b Clustered column plots specifying the number of H3K36me2&3-enriched genes (i.e. log₂ ChIP/input ≥ 0.2 and ≥ 0.15, respectively) with genes robustly enriched (i.e. log₂ ChIP/input ≥ 0.5) represented by patterned bars. C Numbers of genes with H3K36me2&3 enrichment (MACS2 peaks, q-value < 0.05; present in both replicates) in stage II and post-stage II gametocytes. No peaks were detected for pre-stage II gametocytes. The proportion of genes with enrichment 1.5 kb upstream of TSSs is represented by the darker shade compared to the proportion of genes with enrichment in the coding regions (CDRs). d Log₂-transformed ChIP/input ratio tracks for H3K36me2&3 in stage II and post-stage II gametocytes for a region on chromosome 8 to exemplify significant enrichment (MACS2 peaks present in both biological replicates, q-value < 0.05) in stage II gametocytes. Called peaks are indicated by the horizontal black bars