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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Mechanism and functional role of the interaction between CP190 and the architectural protein Pita in Drosophila melanogaster

Fig. 6

CP190 is required for Pita boundary activity. a A schematic showing the regulatory regions of the Abd-B gene. The green arrow indicates the Abd-B gene. The iab-domains (iab-5–iab-8) are separated by boundaries (Mcp, Fab-6, Fab-7, and Fab-8) that are shown by vertical black bars. Below, a schematic representation of the Fab-7 boundary replacements at the Fab-7attP50 deletion. The HS*, HS1, HS2, and HS3 hypersensitive sites are indicated as grey boxes. The Fab-7attP50 deletion contains an attP site for transgene integration and lox- and frt-sites for the excision of the reporter genes and plasmid sequences. b The morphologies of abdominal segments (numbered) in males carrying different combinations of mutations. The red arrows show the signs of a gain-of-function (GOF) phenotype (transformation of the A6 segment into a copy of A7). The blue arrows show the signs of a loss-of-function (LOF) transformation (transformation of the A6 segment into a copy of A5) that is directly correlated with the boundary functions of tested DNA fragments. In Fab-7attP50 males, A6 transforms into A7 (GOF), which leads to the absence of a corresponding segment. In wt males, the A5 sternite has a quadrangular shape and is covered with bristles, whereas the A6 sternite has a distinctly concave, elongated shape and lacks bristles. In Pita×5 males, the A6 segment is transformed into a copy of A5: both sternites have a quadrangular shape and are covered with bristles. pita/CyO and pita indicate pitak05606/CyO and pita02132/pitak05606, respectively. c Morphologies of the abdominal segments (numbered) in Pita×5 males expressing Ubi:Pitawt or Ubi:PitaΔCP1 in the wild-type or pita (pita02132/pitak05606) background. d Compared with the binding of FLAG-Pita and CP190, the binding region in males expressing Ubi:Pitawt or Ubi:PitaΔCP1 were assessed in the wild-type or pita background. Histograms show ChIP enrichments at the Pita×5 region on chromatin isolated from males expressing different variants (wt and lacking the CP190-binding region) of Pita protein. The results are presented as a percentage of input genomic DNA, normalized to the corresponding positive autosomal genome region at the 100C cytological locus. Error bars show standard deviations of triplicate PCR measurements for two independent experiments. Asterisks indicate significance levels: *p < 0.05, **p < 0.01

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