Fig. 1.

Mapping the CP190-interacting regions in the Pita protein. a Schematic representation of full-length Pita protein showing the CP190-binding regions (gray boxes). The positions of the amino acid residues are shown at the top of the panel. b GST- and 6×His-pull-down of GST-fused Pita protein fragments co-expressed with the thioredoxin-6×His-fused CP190 BTB domain. The positions of the amino acids are given in square brackets. c Schematic summary of the pull-down results. d Multiple sequence alignment of the CP190 BTB-domain-interacting peptide in Pita protein from various Drosophila species shows the high conservation of hydrophobic and positively charged residues. Residue numbers above the alignment are for D. melanogaster Pita protein.