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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids

Fig. 1

H1T2 possess highly divergent C terminal domain. a Multiple sequence alignment of sequences of linker histone variants. Sequences from either somatic linker histone H1d or germ cell-specific H1t were aligned to consensus sequence from germ cell-specific H1T2 of different species. Amino acids are colored according to their similarity degree using the ClustalX option of Jalview. b Schematic representation of domain architecture of rat H1T2 protein: Globular domain (red), ATP-binding walker motif (green), serine-arginine rich domain (blue). Peptide sequence against which the antibody is raised, is highlighted in the C terminal domain. Clustal alignment of H1T2 sequence from rat, mouse and human species is also shown in the figure. Conserved globular domain, walker motif (ATP binding), and the divergent SR domain in the H1T2 sequences are represented separately. c Coiled-coil prediction analysis of rat H1T2 sequence (https://embnet.vital-it.ch/cgi-bin/COILS_form_parser). d Secondary structure of the rat H1T2 sequence predicted using Phyre2 (PDB format)

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Epigenetics & Chromatin

ISSN: 1756-8935