Fig. 1

UTX binding correlates with promotion and suppression of gene expression in hNSCs. a UTX changes most of its target genes during the differentiation of hESCs to hNSCs. b Quantification of UTX transcript level in hESCs, hNSCs, and neurons. ***P < 0.001 by one-sided Student’s t test. c Gene ontology analysis of UTX-bound genes in hNSCs. Ontology terms were ranked by P value significance, with the number of enriched genes indicated. d CRISPR sgRNA sequences and mutations in UTX-KO clones. Orange sequences indicate sgRNA targets, dots indicate deletion, the blue “A” indicates an insertion, and “al” denotes allele. e WB analysis of UTX-WT cells and UTX-KO clones. f IF of neural differentiation markers in UTX-WT hESCs, UTX-WT hNSCs, and UTX-KO cells. Bar, 50 μm. g UTX-bound genes in hNSCs were enriched with differentially expressed genes in UTX-KO vs. UTX-WT differentiating cells. P values were calculated by the hypergeometric test, assuming normal data distribution