Fig. 6

Distribution of dADD1 on heterochromatic genes and effects of RNAi-mediated dADD1 knockdown a Comparison between the expression levels of heterochromatic genes and occupancy of H3K9me3, H3K36me3, HP1a and ADD1 on the gene body across heterochromatic genes with no, low, moderate and high expression. Heterochromatic gene expression is associated with an increased enrichment of dADD1 at the TSS and H3K36me3 at the gene body. b RT-qPCR data showing the fold change in expression (normalized to Rp49) upon RNAi-mediated dADD1 knockdown compared to the control (n = 2, Mean + SE). Euchromatic genes in green and heterochromatic genes in red. c Comparison of the enrichment of H3K36me3 marks uisng ChIP-qPCR in WT and dADD1 knockdown conditions (n = 2, Mean + SE)