Fig. 4

DNA methylation regulates the expression of BmCHT10 via Bmara. a. DNA pull-down experiment with the wild type or mutated − 250 to − 225 nt fragment of the BmCHT10 promoter (DNA probe) and the nuclear proteins isolated from the wing disc of 3-day-old pupae. b Effects of 5-aza-dC treatment on the mRNA (left) and protein (right) levels of Bmara at pupal stages. c Effects of Bmara RNAi on the luciferase activities of the different promoter truncates of BmCHT10 in Bm12 cells: − 1100 to − 1 nt, − 900 to − 1 nt, − 500 to − 1 nt, − 300 to − 1 nt, − 250 to − 1 nt and − 225 to − 1 nt promoter fragments. d Effects of Bmara overexpression on the reporter luciferase activity under the control of the − 250 to − 225 nt fragment of BmCHT10 promoter in the Bm12 cells. e Effects of Bmara RNAi on the reporter luciferase activity under the control of the − 250 to − 225 nt fragment of BmCHT10 promoter in the Bm12 cells. f Effects of 5-aza-dC treatment or BmDnmt1 RNAi on the luciferase activity of − 250 to − 225 nt promoter fragment after Bmara RNAi. Each data point is the mean ± SE of three independent assays. For the t test: p < 0.05 (*) or p < 0.01(**)